| 產品編號 | bs-3582R-FITC |
| 英文名稱 | Rabbit Anti-TIF1 gamma/FITC Conjugated antibody |
| 中文名稱 | FITC標記的轉錄中介因子Tif1γ抗體 |
| 別 名 | Trim33; TIF1 gamma;RFG7; Rfg7 protein; TF1G; TIF1-gamma; TIF1G; TIF 1G; TIF1GAMMA; TIFGAMMA; Transcription intermediary factor 1-gamma; Transcriptional intermediary factor 1 gamma; Trim 33; Tripartite motif containing 33; Tripartite motif containing 33 protein; tripartite motif-containing 33; Tripartite motif-containing protein 33; E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; Protein Rfg7; PTC7 antibody; Ret fused gene 7; RET-fused gene 7 protein; RFG7; KIAA1113; TRI33_HUMAN; Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; Transcription intermediary factor 1-gamma. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 腫瘤 免疫學 信號轉導 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Cow, Horse, ) |
| 產品應用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 124kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TIF1 gamma(987-1048aa) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: TIF1 gamma is a member of the Transcriptional Intermediary Factor 1 family, which serve as coactivators and corepressors. Its structure is similar to TIF1 alpha and TIF1 beta, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain). TIF1 gamma functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells. Function: Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade). Subunit: Homooligomer and heterooligomer with TRIM24 and TRIM28 family members. Interacts with SMAD4 in unstimulated cells. Found in a complex with SMAD2 and SMAD3 upon addition of TGF-beta. Interacts with SMAD2 and SMAD3. Interacts with SMAD4 under basal and induced conditions and, upon TGF-beta signaling, with activated SMAD2. Forms a ternary complex with SMAD4 and SMAD2 upon TGF-beta signaling. Subcellular Location: Nucleus. Note=In discrete nuclear dots resembling nuclear bodies. Tissue Specificity: Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors. DISEASE: Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM33 is found in thyroid papillary carcinomas. Translocation t(1;10)(p13;q11) with RET. The translocation generates the TRIM33/RET (PTC7) oncogene. Similarity: Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 bromo domain. Contains 1 PHD-type zinc finger. Contains 1 RING-type zinc finger. Database links: Entrez Gene: 51592 Human Entrez Gene: 94093 Mouse Omim: 605769 Human SwissProt: Q9UPN9 Human SwissProt: Q99PP7 Mouse Unigene: 26837 Human Unigene: 195036 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
