| 產品編號 | bsm-63265R |
| 英文名稱 | phospho-Tuberin (T1462) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化馬鈴薯球蛋白(結節性硬化)重組兔單抗 |
| 別 名 | LAM; PPP1R160; Protein phosphatase 1; regulatory subunit 160; tsc2; TSC4; TSC4; formerly; Tuberin; Tuberous sclerosis 2. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 6F3 |
| 交叉反應 | Human,Mouse |
| 產品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 201 kDa |
| 檢測分子量 | 201 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Tuberin around the phosphorylation site of T1462: PRGY-pT-ISD |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. SWISS: P49815 Gene ID: 7249 Tuberin 為180kD-220的蛋白產生于結節性硬化（TSC-2）位于染色體16的基因。結節性硬化為常染色體疾病已知為斑痣性錯構瘤病其特征為廣泛性發展呈良性生長，在許多組織與器官中描述為錯構瘤。Tuberin被認為在GTPase活動性蛋白中起作用調節細胞內吞與作為腫瘤抑制子。Tuberin低水平廣泛性表達在大多數組織中，而在皮層神經元、小腦浦肯野氏細胞、脊索運動神經元、胰島B細胞、心肌、腎臟與皮膚的小血管中表達增加。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
