| 產品編號 | bsm-63216R |
| 英文名稱 | SPHK1 Recombinant Rabbit mAb |
| 中文名稱 | 鞘氨醇激酶1重組兔單抗 |
| 別 名 | SK1; Sphingosine kinase 1; SPHK; Sphk1; SPK. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 3E7 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,Flow-Cyt=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43 kDa |
| 檢測分子量 | 51 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human SK 1: 40-75 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. SWISS: Q9NYA1 Gene ID: 8877 SPHK1參與鞘氨醇代謝、G蛋白偶聯受體蛋白信號轉導途徑，有抗細胞凋亡的作用，可被蛋白激酶C激活，參與細胞內信號轉導級聯、鈣介導信號轉導、調控細胞生長遷移、調控血管生成及成纖維細胞增殖。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
