| 產品編號 | bsm-63195R |
| 英文名稱 | MITF Recombinant Rabbit mAb |
| 中文名稱 | 微小細胞血癥相關轉錄因子重組兔單抗 |
| 別 名 | Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32; MITF; BHLHE32. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 7B10 |
| 交叉反應 | Human,Rat (predicted: Mouse) |
| 產品應用 | IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 59 kDa |
| 檢測分子量 | 52 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MITF: 350-526 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR). SWISS: O75030 Gene ID: 4286 MITF微小轉錄因子是一個黑色素細胞的核蛋白,對黑色素細胞的生成和活性起著關鍵作用,MITF也是控制細胞外信號的一項調節因子。MITF高度表達于原發和轉移的惡性黑色素瘤，也可視為高敏感和高特異的黑色素細胞標記。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MITF Monoclonal Antibody, Unconjugated (bsm-63195R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MITF Monoclonal Antibody, Unconjugated (bsm-63195R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
