| 產品編號 | bsm-54033R |
| 英文名稱 | smooth muscle Myosin heavy chain 11 Recombinant Rabbit mAb |
| 中文名稱 | 平滑肌肌球蛋白重鏈抗體 |
| 別 名 | SMMHC; Myosin 11; Myosin-11; Myosin, smooth muscle, heavy chain; Myosin Smooth Muscle; AAT 4; AAT4; DKFZp686D10126; DKFZp686D19237; FAA 4; FAA4; FLJ35232; KIAA0866; MGC126726; MGC32963; MYH 11; MYH11 protein; Myosin heavy chain 11 smooth muscle; Myosin heavy chain smooth muscle isoform; Myosin heavy polypeptide 11 smooth muscle; Myosin11; SMHC; Smooth muscle myosin heavy chain isoform SM1; MYH11_HUMAN. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 1F11 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 227 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human Myosin heavy chain 11: 2-34 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16[inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified.
MYH11 is a smooth muscle myosin belonging to the myosin heavy chain family. It is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. SWISS: P35749 Gene ID: 4629 平滑肌肌球蛋白重鏈是分子量為200kDa的多肽，是六聚肌球蛋白的結構成分。主要用于標記血管和內臟平滑肌細胞以及肌上皮細胞，被認為是平滑肌較為特異和可靠的標志，有助于間葉腫瘤的診斷和分類，也可用于乳腺肌上皮細胞的檢測，有助于原位癌和浸潤癌的區別鑒定。 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with smooth muscle Myosin heavy chain 11 monoclonal antibody, unconjugated (bsm-54033R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with smooth muscle Myosin heavy chain 11 Monoclonal Antibody, Unconjugated(bsm-54033R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
