| 產品編號 | bsm-60866R |
| 英文名稱 | Citrulline-Histone H3 (Arg2/8/17) Recombinant Rabbit mAb |
| 中文名稱 | 瓜氨酸組蛋白3重組兔單抗 |
| 別 名 | H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3.1; H3/d; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C7; H3C8; H3FD; H3R2/R8/R17cit; 瓜氨酸化組蛋白3; 瓜氨酸組蛋白H3; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-100,IHC-F=1:50-100,IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | PBS, Glycerol, BSA. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. 產品組分及規格 編號 組分 規格 濃度 儲存 1 |
| 產品圖片 |
Sealing solution and concentration: 5% NFDM/TBST
Dilution of primary antibody: 1:2000
Incubation conditions for primary antibody: 2 hours at room temperature
Immunogen peptide sample size: 1:1 ng, 4 ng, 16 ng, 64 ng, 2-6:64ng
Exposure time: 10 seconds
Sealing solution and concentration: 5% NFDM/TBST
Dilution of primary antibody: 1:20000
Incubation conditions for primary antibody: 2 hours at room temperature
Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP)
Cracking solution: (-): HL-60, (+): HL-60 + DMSO + A23187 (4uM,15min)
Protein loading amount: 20 μ g
Exposure time: 60 seconds
Theoretical molecular weight: 15 kDa
Actual molecular weight: 15 kDa
Sealing solution and concentration: 5% NFDM/TBST
Dilution ratio of primary antibody: 1:100000
Incubation conditions for primary antibody: 2 hours at room temperature
Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP)
Cracking solution: (Lane 1) 293T transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 μM Ionomycin for 2 hours, whole cell lysate; (Lane 2) 293T transfected with GFP-tagged PADI4 (WT, Q9UM07) expression vector, treated with 10 mM calcium chloride and 10 μM Ionomycin for 2 hours, whole cell lysate.
Protein loading amount: 20 μ g
Exposure time: 10 seconds
Theoretical molecular weight: 15 kDa
Actual molecular weight: 15 kDa
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Antibody incubation with Citrulline-Histone H3 (Arg2/8/17) Monoclonal Antibody, Unconjugated(bsm-60866R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Negative control： site-specific peptide blocking
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
