產品編號 | bsm-60865R |
英文名稱 | Acetyl Lysine Recombinant Rabbit mAb |
中文名稱 | 乙酰基賴氨酸/乙酰化賴氨酸重組兔單抗 |
別 名 | Acetylated Lysine; Acetyllysine; Ac-lysine; Ac lysine; Ac-K; Ac K; Ac-Lys; Ac Lys; acetyl-Lysine; acetyl Lys; Ac-Lysine; 賴氨酸乙酰化; 乙酰化修飾賴氨酸;乙酰賴氨酸; |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | 19D12 |
交叉反應 | Species independent |
產品應用 | WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | kDa |
檢測分子量 | |
細胞定位 | 細胞核 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | PBS, Glycerol, BSA. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
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產品圖片 |
Sealing solution and concentration: 5% NFDM/TBST
First antibody dilution: 1:1000
Incubation conditions for primary antibody: 2 hours at room temperature
Cracking solution: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Mouse liver, 5: Mouse kidney
Secondary antibody: Goat Anti Rabbit IgG H&L (HRP)
Protein loading amount: 20 μ G
Exposure time: 60 seconds
Theoretical molecular weight: Multiple kDa
Actual molecular weight: Multiple kDa
Tissue: mouse brain
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60865R
Tissue: rat liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60865R
Tissue: human liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60865R
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |