| 產品編號 | bsm-56205R |
| 英文名稱 | IL18 Recombinant Rabbit mAb |
| 中文名稱 | 白細胞介素-18/干擾素γ誘導因子重組兔單抗 |
| 別 名 | Interleukin-18; interferon gamma-inducing factor IGIF; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; Iboctadekin; IFN gamma inducing factor; IGIF; IL 1 gamma; IL 18; IL-18; IL 1g; IL1 gamma; IL18 protein; IL1F4; IL1g; IL1gamma; ILIF4; Interferon gamma inducing factor; Interleukin 1 gamma; Interleukin18; MGC12320; IL18_HUMAN. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 14E15 |
| 交叉反應 | Human |
| 產品應用 | WB=1:1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human IL18 protein: 1-192/192 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | PBS (pH7.2) with 40% Glycerol, 0.05% BSA and 0.02% Proclin300. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. Function: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Subcellular Location: Secreted. Similarity: Belongs to the IL-1 family. SWISS: Q14116 Gene ID: 3606 Database links: Entrez Gene: 3606 Human
Entrez Gene: 16173 Mouse
Omim: 600953 Human
SwissProt: Q14116 Human
SwissProt: P70380 Mouse
Unigene: 83077 Human
Unigene: 1410 Mouse
IL-18是巨噬細胞分泌的一個重要因子，它具有多種免疫功能，能誘導產生ＩＦＮγ，無論在病毒感染早期或特異性細胞免疫應答效應階段都會出現，因此IL-18主要參與免疫應答及炎性細胞浸潤的改變。Interleukin-18 又稱inteferon-gamma inducing factor(IGIF)，在輔助T細胞的應答反應中起重要作用。IL-18和IL-1一起，既參與先天免疫，又是后天免疫的構成因素。 |
| 產品圖片 |
Sample:
Lane 1: Normal HeLa cell lysates
Lane 2: HeLa cell treated with Brefeldin A (5 μ g/ml, 15 min)
Lane 3: Jurkat cell lysates (Negative control )
Primary: Anti-IL18 (bsm-56205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 23 kDa
Paraformaldehyde-fixed, paraffin embedded (Human spleen); Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (IL18) Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:250 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (IL18) Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:250 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (IL18) Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:250 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
