產品編號 | bsm-34317R |
英文名稱 | CREB Recombinant Rabbit mAb |
中文名稱 | 環腺苷酸應答元件結合蛋白重組兔單抗 |
別 名 | Active transcription factor CREB; cAMP response element binding protein; cAMP responsive element binding protein 1; CREB 1; CREB1; CREB-1; MGC9284; Transactivator protein; CREB; CREB1_HUMAN. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | 14D4 |
交叉反應 | Human,Mouse (predicted: Rat) |
產品應用 | WB=1:500-1000,IHC-P=1:50-100,IHC-F=1:50-100,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 37 kDa |
檢測分子量 | |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CREB |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | pH7.4, 150mM NaCl, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 | |
產品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human HepG2 cell lysates
Lane 5: Human SH-SY5Y cell lysates
Primary: Anti-CREB (bsm-34317R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 46 kDa
|
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |