| 產品編號 | bsm-60743R |
| 英文名稱 | Chk1 Recombinant Rabbit mAb |
| 中文名稱 | 細胞周期檢測點激酶1重組兔單抗 |
| 別 名 | Cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog(S. pombe); CHEK 1; CHEK-1; CHEK1; Chk 1; CHK1 checkpoint homolog(S. pombe); EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A(S. cerevisiae); C85740; CHK1_HUMAN. |
| 研究領域 | 腫瘤 染色質和核信號 細胞周期蛋白 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 7D2 |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Chk1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 50mM Tris-Glycine (pH7.4), 0.15M NaCl, 40% Glycerol, 0.02% Proclin300 and 0.05% BSA. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2011]. SWISS: O14757 Gene ID: 1111 Database links: Entrez Gene: 1111 Human Entrez Gene: 12649 Mouse Omim: 603078 Human SwissProt: O14757 Human SwissProt: O35280 Mouse Unigene: 24529 Human Unigene: 16753 Mouse Unigene: 33267 Rat |
| 產品圖片 |
Sample:
Lane 1: Human A431 cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human MOLT4 cell lysates
Lane 4: Human MCF-7 cell lysates
Lane 5: Human U-2 OS cell lysates
Primary: Anti-Chk1 (bsm-60743R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
