| 產品編號 | bsm-60443R |
| 英文名稱 | PTGER2 Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | C11G4 |
| 交叉反應 | Human (predicted: Mouse) |
| 產品應用 | WB=1:500-1:1000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | 半胱氨酸蛋白酶，細胞質到液泡運輸（Cvt）和自噬所需的半胱氨酸蛋白酶。將ATG8家族蛋白MAP1LC3、GABARAPL1、GABARAPL2和GABARAP的C端氨基酸裂解，從而揭示C端甘氨酸。在C端暴露甘氨酸對ATG8蛋白與磷脂酰乙醇胺（PE）結合和膜的插入是必不可少的，而這是自噬所必需的。 |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HepG2, Jurkat
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 48 kDa
Observed MW: 48 kDa
Cell line: NIH-3T3
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60443R
Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60443R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
