| 產品編號 | bsm-60080M |
| 英文名稱 | Phospho-Histone H3 (Ser10) Recombinant Mouse mAb |
| 中文名稱 | 磷酸化組蛋白H3單克隆抗體 |
| 別 名 | Histone H3(phospho S10); phospho-Histone H3(Ser10); Histone H3(phospho-Ser10); Histone H3(p-Ser10); Histone H3(phospho-S10); H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H31_HUMAN. |
| 產品類型 | 磷酸化抗體 |
| 抗體來源 | Mouse |
| 克隆類型 | Recombinant |
| 克 隆 號 | C10C6 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-1:1000,IHC-P=1:100-500,ICC/IF=1:50-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser10: RK(p-S)TG |
| 亞 型 | IgG1/Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] Subcellular Location: Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC). SWISS: Q71DI3 Gene ID: 8968 組蛋白翻譯后修飾（PTMs）是表觀遺傳學調控染色質結構的關鍵機制，被稱為“組蛋白密碼”。組蛋白上的翻譯后修飾包括乙酰化，甲基化，磷酸化和近年發現的一些新型酰化修飾。這些組蛋白修飾直接影響染色質和轉錄因子或其他表觀調控子的結合，改變基因組的穩定性和基因轉錄等。組蛋白磷酸化通常發生在核心組蛋白N端的絲氨酸，蘇氨酸或酪氨酸上，在DNA修復，轉錄和染色質折疊等方面起著重要作用。廣為人知的組蛋白磷酸化位點為H2A.xS139ph，有文獻報道此位點與DNA損傷相關。組蛋白磷酸化主要參與有絲分裂和減數分裂過程。參與調控磷酸化水平的激酶與磷酸酶多種多樣。 |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+ Nocodazole (100ng/ml, 18hr)
Protein loading quantity: 20 μg
Tissue: Rat testis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room tem
Tissue: Mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room te
Tissue: Human kidney
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room t
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
