| 產品編號 | bsm-60618R |
| 英文名稱 | SATB1 Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | H6D4 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-1:2000,IHC-P=1:100-500,Flow-Cyt=1:20-1:100,ICC/IF=1:20-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | 促成 Xist RNA 介導的 X 失活啟動的關鍵沉默因子，發生在胚胎發生和淋巴瘤中。在富含 AT 的特殊序列（共有 SATB1 結合序列 (CSBS)，在核基質或支架相關區域）與 DNA 結合。認為識別雙鏈 DNA 的糖磷酸結構。通過與 DNA 的基質附著區 (MAR) 結合并誘導局部染色質環重塑，以磷酸化和乙酰化狀態依賴性方式控制核和病毒基因表達的轉錄抑制因子。作為幾種染色質重塑酶（例如 MHC-I 位點的 PML）的停靠位點，還可以直接向啟動子和增強子募集輔抑制因子 (HDAC) 或輔激活因子 (HAT)。調節對來自胸腺細胞的免疫 T 細胞 CD8SP 的成熟至關重要的基因。需要轉換胎兒珠蛋白種類，以及在紅細胞分化過程中調節 β 和 γ 珠蛋白基因。 |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: Jurkat, 2: THP-1
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 86 kDa
Observed MW: 100 kDa
Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60618R
Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60618R
Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60618R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
