| 產品編號 | bsm-60528M |
| 英文名稱 | WNT10B Recombinant Mouse mAb |
| 中文名稱 | 信號通路WNT10B重組鼠單抗 |
| 別 名 | WN10B_HUMAN; SHFM6; wingless type MMTV integration site family, member 10B; wnt12; WNT10-B. |
| 研究領域 | 腫瘤 免疫學 信號轉導 轉錄調節因子 |
| 抗體來源 | Mouse |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-1:100,IHC-F=1:400-800,IF=1:100-500,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WNT10B |
| 亞 型 | IgG1/Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008] SWISS: O00744 Gene ID: 7480 |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:10000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: SH-SY5Y, 3: C2C12
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 43 kDa
Observed MW: 43 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT10B) Monoclonal Antibody, Unconjugated (bsm-60528M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Tissue: Human liver cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60528M
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60528M
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
