| 產品編號 | bsm-30078M-APC |
| 英文名稱 | human CD33-APC |
| 中文名稱 | APC標記人CD33單克隆抗體 |
| 別 名 | CD33_HUMAN; CD 33; CD33 antigen; CD33 molecule; FLJ00391; gp67 ; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67 ; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-3; myeloid cell surface antigen CD33 isoform 1 precursor. APC anti-human CD33; |
| 研究領域 | 免疫學 干細胞 細胞表面分子 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | HI33a |
| 交叉反應 | |
| 產品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40/37kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 亞 型 | IgG2a |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M PBS, 0.5%BSA, 0.03%Proclin300 |
| 保存條件 | Store at 2-8℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Subunit: Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Monocytic/myeloid lineage cells. Post-translational modifications: Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P20138 Gene ID: 945 Database links: Entrez Gene: 945 Human Omim: 159590 Human SwissProt: P20138 Human Unigene: 83731 Human |
| 產品圖片 |
Flow cytometry staining of normal human peripheral blood cells with CD33/APC(bsm-30078M-APC)(Red histogram) or Mouse IgG2a Isotype Control (APC Conjugate)(C03-12004)(Blue histogram) . Total cells were used for analysis.
Blank control:U-937.
Primary Antibody (green line): Rabbit Anti-CD33 antibody (bsm-30078M-APC)
Dilution: 1ug/Test;
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
