| 產品編號 | bs-20294R |
| 英文名稱 | LDHB Rabbit pAb |
| 中文名稱 | 乳酸脫氫酶抗體 |
| 別 名 | Lactate Dehydrogenase B; Renal Carcinoma Antigen NY-REN-46; LDH Heart Subunit; EC 1.1.1.27; LDH-B; LDH-H; Epididymis Secretory Protein Li 281; Lactate Dehydrogenase H Chain; HEL-S-281; EC 1.1.1; LDHBD; TRG-5; LDHB_HUMAN; |
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Specific References (1) | bs-20294R has been referenced in 1 publications.
[IF=4.486] Du Y et al. ATPR triggers acute myeloid leukaemia cells differentiation and cycle arrest via the RARα/LDHB/ERK‐glycolysis signalling axis. J Cell Mol Med. 2020 Jun; 24(12): 6952–6965. IHC ; mouse.
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| 研究領域 | 細胞生物 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LDHB: 181-250/334 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Recent studies have shown that two protein isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism, and that these C-terminally distinct isoforms have different subcellular localization. Alternatively spliced transcript variants have also been found for this gene. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Oct 2015] Function: This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation. View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation. Subunit: Homotetramer. Subcellular Location: Cytoplasm Similarity: Belongs to the LDH/MDH superfamily. LDH family. SWISS: P07195 Gene ID: 3945 Database links: Entrez Gene: 3945 Human Entrez Gene: 16832 Mouse Omim: 150100 Human SwissProt: P07195 Human SwissProt: P16125 Mouse Unigene: 446149 Human Unigene: 9745 Mouse Unigene: 1785 Rat |
| 產品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Jurkat (Human) Lysate at 40 ug
Primary: Anti-LDHB (bs-20294R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
