| 產品編號 | bs-20028R |
| 英文名稱 | MTA3 Rabbit pAb |
| 中文名稱 | 轉移相關蛋白MTA3抗體 |
| 別 名 | 1110002J22Rik; fj99h01; KIAA1266; Metastasis associated 1 family, member 3; Metastasis associated 3; Metastasis associated family, member 3; Metastasis associated gene 3; Metastasis associated gene family, member 3; Metastasis associated protein MTA3; Metastasis-associated protein MTA3; MGC56396; MGC77410; mKIAA1266; mta1; Mta3; MTA3 metastasis associated 1 family, member 3; Mta3 metastasis associated 3; MTA3_HUMAN; wu:fj99h01; zgc:56396. |
| 研究領域 | 細胞生物 信號轉導 生長因子和激素 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 67 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MTA3: 501-594/594 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Metastasis-associated protein 3 (MTA3) is a subunit of the Mi-2/NuRD transcriptional corepressor complex. MTA3 and the Mi-2/NuRD complex mediate repression of Snail in breast cancer cells where MTA3 works to maintain a differentiated, epithelial status. The protein is involved in gene expression regulation by covalent modifications of histone proteins. There are two known isoforms of MTA3, a short and a long form. The short isoform binds to ER and sequesters it to the cytoplasm and betters non-genomic responses, whereas the long form is found in the nucleus. MTA3 is widely expressed in with highest expression in brain, adrenal glands, ovaries and virgin mammary glands. It has been found to be expressed in higher levels in tumors than in adjacent normal tissue in the same individual. Function: Plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels. Subcellular Location: Nucleus. Similarity: Contains 1 BAH domain. Contains 1 ELM2 domain. Contains 1 GATA-type zinc finger. Contains 1 SANT domain. SWISS: Q9BTC8 Gene ID: 57504 Database links: Entrez Gene: 57504 Human Entrez Gene: 116871 Mouse Omim: 609050 Human SwissProt: Q9BTC8 Human SwissProt: Q924K8 Mouse Unigene: 435413 Human Unigene: 277668 Mouse Unigene: 94848 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTA3) Polyclonal Antibody, Unconjugated (bs-20028R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTA3) Polyclonal Antibody, Unconjugated (bs-20028R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
