| 產品編號 | bs-12882R |
| 英文名稱 | BPY2 Rabbit pAb |
| 中文名稱 | 睪丸特異性堿性蛋白Y2抗體 |
| 別 名 | Basic charge; Basic charge Y linked 2; Basic charge Y linked 2C; Basic protein on Y chromosome 2; BPY2A; BPY2B; BPY2C; Testis specific basic protein on Y 2; Testis specific basic protein Y 2; Testis-specific basic protein Y 2; Variable charge Y linked 2; Variably charged protein Y 2; VCY2; VCY2_HUMAN; VCY2A; VCY2B; VCY2C; Y-linked 2. |
| 研究領域 | 細胞生物 發育生物學 干細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BPY2: 51-106/106 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Mammalian spermatogenesis is a complex developmental process. Mutations at multiple loci and in structurally and functionally disparate genes in the genome affect gametogenesis. The analysis of mutations has provided insight into biochemical pathways required for completion of this process. The basic protein on Y chromosome 2 gene (BPY2, also designated VCY2) is located in a frequently deleted azoospermia factor c region. Three copies (paralogs) of the BPY2 gene (BPY2A, BPY2B, BPY2C) reside in the AZFc region. BPY2 protein expression is localized to the nuclei of spermatogonia, spermatocytes and round spermatids, but is absent from elongated spermatids. Impaired expression of BPY2 in infertile men suggests its involvement in male germ cell development. BPY2 interacts with MAP-1S, which shares homology with microtubule-associated proteins (MAPs), suggesting a role for BPY2 within the cytoskeletal network. Subunit: Interacts with MAP1S. Interacts with UBE3A (via HECTdomain). Tissue Specificity: Expressed exclusively in testis. Expressed in ejaculated spermatozoa of germ cell. Expressed in the nuclei of spermatogonia, spermatocytes, and round spermatids, except elongated spermatids (at protein level). Similarity: Belongs to the VCX/VCY family. SWISS: O14599 Gene ID: 442867 Database links:
Entrez Gene: 442867 Human SwissProt: O14599 human |
| 產品圖片 |
Sample: HL-60 Cell (Human) Lysate at 40 ug
Primary: Anti-BPY2 (bs-12882R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kD
Observed band size: 12 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
