| 產品編號 | bs-9594R |
| 英文名稱 | PINX-1 Rabbit pAb |
| 中文名稱 | 端粒酶抑制因子PinX-1抗體 |
| 別 名 | 67-11-3 protein; FLJ20565; Hepatocellular carcinoma-related putative tumor suppressor; Liver-related putative tumor suppressor; LPTL; LPTS; MGC8850; PIN2 interacting protein 1; Pin2-interacting protein X1; PIN2/TERF1-interacting telomerase inhibitor 1; PINX1; PINX1_HUMAN; Protein 67-11-3; TRF1-interacting protein 1. |
| 研究領域 | 腫瘤 細胞生物 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Pig,Cow,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PINX-1: 65-160/328 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
PINX1 is a ubiquitously expressed protein that localizes to nucleoli and telomere speckles. PINX1 contains one G-patch domain and one telomeric inhibiting domain (TID) at its C-terminus. PINX1 interacts with the telomere protein TRF1 and the telomerase reverse transcriptase TERT. The TID domain of PINX1 specifically interacts with TERT and functions to inhibit its activity, thus participating in the regulation of telomerase activity. Overexpression of PINX1 leads to shortened telomeres, further supporting an inhibitory role of PINX1 on telomerase activity. The depletion of PINX1 significantly increases telomerase activity and may lead to tumorigenicity of cancer cells. This suggests that PINX1 acts as a tumor suppressor and can inhibit cell proliferation. In addition, PINX1 is involved in nucleolar RNA maturation. Function: Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor. Subunit: Interacts with MCRS1, TERT, TERF1, NCL/nucleolin, and the telomerase RNA. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous; expressed at low levels. Similarity: Belongs to the PINX1 family. Contains 1 G-patch domain. SWISS: Q96BK5 Gene ID: 54984 Database links: Entrez Gene: 54984 Human Entrez Gene: 72400 Mouse Omim: 606505 Human SwissProt: Q548A5 Human SwissProt: Q6QWG9 Human SwissProt: Q96BK5 Human SwissProt: Q9CZX5 Mouse Unigene: 490991 Human Unigene: 583894 Human Unigene: 379214 Mouse Unigene: 15365 Rat |
| 產品圖片 |
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PINX-1 Polyclonal Antibody, Unconjugated(bs-9594R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-PINX-1 antibody (bs-9594R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
