| 產品編號 | bs-9762R |
| 英文名稱 | FGF18 Rabbit pAb |
| 中文名稱 | 成纖維細胞生長因子18抗體 |
| 別 名 | FGF 18; FGF18; FGF-18; Fibroblast Growth Factor 18; ZFGF5; FGF18_HUMAN. |
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Specific References (2) | bs-9762R has been referenced in 2 publications.
[IF=11.501] WB ;
[IF=6.384] Anthony Estienne. et al. Endothelial cell-derived fibroblast growth factor-18 regulates ovarian function in sheep. 2022 Mar 21 IHC ; Sheep.
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| 研究領域 | 細胞生物 信號轉導 細胞凋亡 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Chicken,Goat) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:200-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FGF18: 110-207/207 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
FGF18 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures. Function: Plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation. Subunit: Interacts with FGFR3 and FGFR4. Subcellular Location: Secreted Similarity: Belongs to the heparin-binding growth factors family. SWISS: O76093 Gene ID: 8817 Database links: Entrez Gene: 8817 Human Entrez Gene: 14172 Mouse Omim: 603726 Human SwissProt: O76093 Human SwissProt: O89101 Mouse Unigene: 87191 Human Unigene: 339812 Mouse Unigene: 44478 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF18) Polyclonal Antibody, Unconjugated (bs-9762R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
