| 產(chǎn)品編號 | bs-8019R |
| 英文名稱 | ICMT Rabbit pAb |
| 中文名稱 | 異戊烯半胱氨酸羧基甲基轉(zhuǎn)移酶抗體 |
| 別 名 | CMT; HSTE14; Icmt; ICMT_HUMAN; Isoprenylcysteine carboxylmethyltransferase; MGC39955; MGC95322; MST098; MSTP098; pcCMT; PCMT; PPMT; Prenylated protein carboxyl methyltransferase; Prenylcysteine carboxyl methyltransferase; Protein-S-isoprenylcysteine O-methyltransferase; RP23-421E12.4; STE14; fcmt. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CMT/Icmt: 211-278/284 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
Tissue specificity:
Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons. Function: Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons. Similarity: Belongs to the isoprenylcysteine O-methyltransferase family. SWISS: O60725 Gene ID: 23463 Database links: Entrez Gene: 23463 Human Entrez Gene: 57295 Mouse Omim: 605851 Human SwissProt: O60725 Human SwissProt: Q9EQK7 Mouse Unigene: 515688 Human Unigene: 277464 Mouse Unigene: 48709 Rat Icmt酶是異戊烯途徑中的一種關(guān)鍵酶，它能將甲基加到Ras上。這種甲基標(biāo)簽?zāi)苁筊as到達(dá)細(xì)胞中的最終目的地，Icmt酶能發(fā)送不控制生長的信號，因此，Icmt蛋白在保持細(xì)胞中的致癌基因Ras的開啟狀態(tài)中起到重要作用。（Ras是細(xì)胞的一種正常的遺傳成分，但是這種基因的突變能夠致使它促進(jìn)細(xì)胞的失控生長。而且，在一半的結(jié)腸癌和90％的胰腺癌中發(fā)生了Ras基因突變）。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICMT) Polyclonal Antibody, Unconjugated (bs-8019R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human pancreatic carcinoma(BXPC-3);4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ICMT Polyclonal Antibody, Unconjugated(bs-8019R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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