| 產品編號 | bs-6314R |
| 英文名稱 | NANOS2 Rabbit pAb |
| 中文名稱 | 生殖細胞增殖相關蛋白NANOS2抗體 |
| 別 名 | NANO2_HUMAN; Nanos homolog 2; Nanos homolog 2 Drosophila; NANOS2; NOS-2; NOS2. |
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Specific References (1) | bs-6314R has been referenced in 1 publications.
[IF=8.079] Honda Naoto. et al. Roles of microglia/macrophage and antibody in cell sheet transplantation in the central nervous system. STEM CELL RES THER. 2022 Dec;13(1):1-11 IHC ; Mouse.
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| 研究領域 | 細胞生物 免疫學 發育生物學 信號轉導 干細胞 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NANOS2: 45-138/138 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Required to support proliferation and self-renewal of proximal germ cells in males only. Probably regulates translation of specific mRNAs by associating with the 3'-UTR of mRNA targets. Essential for spermatogonia formation. Function: Plays a key role in the sexual differentiation of germcells by promoting the male fate but suppressing the female fate.Represses the female fate pathways by suppressing meiosis, which inturn results in the promotion of the male fate. Maintains thesuppression of meiosis by preventing STRA8 expression, which isrequired for premeiotic DNA replication, after CYP26B1 isdecreased. Regulates the localization of the CCR4-NOT deadenylationcomplex to P-bodies and plays a role in recruiting the complex totrigger the degradation of mRNAs involved in meiosis. Required forthe maintenance of the spermatogonial stem cell population. Notessential for the assembly of P-bodies but is required for themaintenance of their normal state (By similarity). Subunit: Interacts with CNOT1, CNOT3, CNOT6L, CNOT7 and CNOT9 (Bysimilarity). Subcellular Location: Cytoplasm. Cytoplasm, P-body (Bysimilarity). Cytoplasm, perinuclear region. Note=Localizes atP-bodies during gonocyte development (By similarity). More abundantin perinuclear region of the cytoplasm of the germ cells of theadult testis. Tissue Specificity: Testis and ovary. Expression found in severalspermatogenic stages: in cells on the periphery of the tubuleswhich could correspond to spermatogonia, in spermatocytes and inround spermatids (at protein level). Similarity: Belongs to the nanos family. Contains 1 nanos-type zinc finger. SWISS: P60321 Gene ID: 339345 Database links: UniProtKB/Swiss-Prot: P60321.1 |
| 產品圖片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-NANOS2 (bs-6314R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 16 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
