| 產品編號 | bs-4065R |
| 英文名稱 | P2X2 Rabbit pAb |
| 中文名稱 | 三磷酸腺苷受體受體P2X2抗體 |
| 別 名 | ATP receptor; P2RX 2; P2RX2; P2RX2_HUMAN; P2X purinoceptor 2; P2X Receptor subunit 2; P2X2; P2X2a; Purinergic receptor; Purinergic receptor P2X ligand gated ion channel 2; Purinergic receptor P2X2 isoform A; Purinergic receptor P2X2 isoform B; Purinergic receptor P2X2 isoform C; Purinergic receptor P2X2 isoform D; Purinergic receptor P2X2 isoform H; Purinergic receptor P2X2 isoform I; MGC129601. |
| 研究領域 | 腫瘤 細胞生物 免疫學 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,GuineaPig (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=2ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 52 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ATP receptor: 251-350/471 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2013] Function: Binding of this ligand-gated ion channel to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Subunit: Functional P2XRs are organized as homomeric and heteromeric trimers. Subcellular Location: Membrane; Multi-pass membrane protein. Similarity: Belongs to the P2X receptor family. SWISS: Q9UBL9 Gene ID: 22953 Database links: Entrez Gene: 22953 Human Entrez Gene: 231602 Mouse Omim: 600844 Human SwissProt: Q9UBL9 Human Unigene: 258580 Human |
| 產品圖片 |
Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-P2X2 (bs-4065R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:52 kD
Observed band size: 67 kD
Blank control:MCF7.
Primary Antibody (green line): Rabbit Anti-P2X2 antibody (bs-4065R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 2μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
