| 產品編號 | bs-5045R |
| 英文名稱 | CPT1B Rabbit pAb |
| 中文名稱 | 肉毒堿棕櫚酰基轉移酶1B抗體 |
| 別 名 | muscle isoform; Carnitine O palmitoyltransferase I mitochondrial muscle isoform; Carnitine O palmitoyltransferase I muscle isoform; Carnitine O-palmitoyltransferase 1, muscle isoform; Carnitine O-palmitoyltransferase I; Carnitine palmitoyltransferase 1A(muscle); Carnitine palmitoyltransferase 1B(muscle); Carnitine palmitoyltransferase 1B; Carnitine palmitoyltransferase I like protein; Carnitine palmitoyltransferase I muscle; Carnitine palmitoyltransferase I-like protein; CPT 1B; CPT I; CPT1 M; CPT1 muscle; CPT1-M; Cpt1b; CPT1B_HUMAN; CPT1M; CPTI; CPTI M; CPTI muscle; CPTI-M; CPTIM; KIAA1670; M CPT1; MCPT1. |
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Specific References (2) | bs-5045R has been referenced in 2 publications.
[IF=4.91] Huebbe, Patricia, et al. "Apolipoprotein E (APOE) genotype regulates body weight and fatty acid utilization–studies in gene targeted replacement mice." Molecular Nutrition & Food Research (2014). WB ; Mouse.
[IF=3.06] Yuan J et al. L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction. Molecules. 2020 Jan 1;25(1). pii: E176. WB ; Mouse.
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| 研究領域 | 腫瘤 細胞生物 免疫學 轉錄調節因子 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | Flow-Cyt=3ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 85 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CPT1B: 501-600/772 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]. Function: Strong expression in heart and skeletal muscle. No expression in liver and kidney. Subcellular Location: Mitochondrion outer membrane. Similarity: Belongs to the carnitine/choline acetyltransferase family. SWISS: Q92523 Gene ID: 1375 Database links: Entrez Gene: 1375 Human Entrez Gene: 12895 Mouse Omim: 601987 Human SwissProt: Q92523 Human SwissProt: Q924X2 Mouse Unigene: 439777 Human Unigene: 609867 Human Unigene: 112195 Human Unigene: 231465 Mouse Unigene: 43113 Rat |
| 產品圖片 |
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-CPT1B antibody (bs-5045R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
