| 產品編號 | bs-3905R |
| 英文名稱 | HSD17B3 Rabbit pAb |
| 中文名稱 | 羥類固醇脫氫酶17β3抗體 |
| 別 名 | 17 beta HSD 3; 17-beta-hydroxysteroid dehydrogenase type 3; EDH17B3; Estradiol 17 beta dehydrogenase; Estradiol 17 beta dehydrogenase 3; Hydroxysteroid(17 beta) dehydrogenase 3; Testicular 17 beta hydroxysteroid dehydrogenase; Testosterone 17 beta dehydrogenase 3; DHB3_HUMAN. |
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Specific References (2) | bs-3905R has been referenced in 2 publications.
[IF=3.585] Sun J et al. Icariin protects mouse Leydig cell testosterone synthesis from the adverse effects of di (2-ethylhexyl) phthalate. Toxicol Appl Pharmacol. 2019 Jun 6;378:114612. ICF&WB ; Mouse.
[IF=1.952] Caiyun Wang. et al. Maternal exposure of mice to sodium p-perfluorous nonenoxybenzene sulfonate causes endocrine disruption in both dams and offspring. 2021 May 11 WB,IHC ; Mouse.
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| 研究領域 | 腫瘤 細胞生物 免疫學 發育生物學 轉錄調節因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HSD17B3: 101-200/310 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
HSD17B3 is involved in the reduction of androstenedione to testosterone. It is expressed predominantly in the testis. Deficiency in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). Function: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Tissue Specificity: Testis. DISEASE: Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH) [MIM:264300]. These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily. SWISS: P37058 Gene ID: 3293 Database links: Entrez Gene: 3293 Human Omim: 605573 Human SwissProt: P37058 Human Unigene: 477 Human |
| 產品圖片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Primary: Anti-HSD17B3 (bs-3905R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HSD17B3 Polyclonal Antibody, Unconjugated(bs-3905R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSD17B3) Polyclonal Antibody, Unconjugated (bs-3905R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
