| 產品編號 | bs-3331R |
| 英文名稱 | Phospho-PFKFB3/PFK2 (Ser467) Rabbit pAb |
| 中文名稱 | 磷酸化果糖-2,6-二磷酸酶3/磷酸果糖激酶2抗體 |
| 別 名 | 6 phosphofructo 2 kinase/fructose 2, 6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2, 6 biphosphatase 3; 6PF 2 K/Fru 2, 6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2, 6 P2ase 3; fructose 6 phosphate, 2 kinase/fructose 2, 6 bisphosphatase; Inducible 6 phosphofructo 2 kinase/fructose 2, 6 bisphosphatase; iPFK 2; IPFK2; PFK/FBPase 3; PFK-2; PFK2; Renal carcinoma antigen NY REN 56; uPFK 2; PFKFB3; F263_HUMAN. |
 |
Specific References (5) | bs-3331R has been referenced in 5 publications.
[IF=4.6] Fu, Wen, et al. "Bioenergetic mechanisms in astrocytes may contribute to amyloid plaque deposition and toxicity." Journal of Biological Chemistry (2015): jbc-M114. Human.
[IF=4.129] Jiang, Bin. et al. Role of Proximal Intestinal Glucose Sensing and Metabolism in the Blood Glucose Control in Type 2 Diabetic Rats After Duodenal Jejunal Bypass Surgery. Obes Surg. 2022 Jan;:1-11 WB ; Rat.
[IF=3.546] Yi,et al.The EMT-related transcription factor snail up-regulates FAPα in malignant melanoma cells.(2018) Experimental Cell Research. 364:160-167. IHC-P ; Human.
[IF=3.231] Lifang Li. et al. Changes in the Expression of MIF and Other Key Enzymes of Energy Metabolism in the Myocardia of Broiler Chickens with Ascites Syndrome. ANIMALS. 2022 Jan;12(19):2488 WB ; Chicken.
[IF=0] Robinson,et al.Role of reactive oxygen species in modulating carbohydrate metabolism in acute myeloid leukaemia. Cardiff University, 2017. WB ; Human.
|
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 免疫學 信號轉導 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human PFK2 around the phosphorylation site of Ser467: LA(p-S)PE |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016] Function: Synthesis and degradation of fructose 2,6-bisphosphate. Tissue Specificity: Ubiquitous. Similarity: In the C-terminal section; belongs to the phosphoglycerate mutase family. SWISS: Q16875 Gene ID: 5209 Database links: Entrez Gene: 5209 Human Entrez Gene: 170768 Mouse Omim: 605319 Human SwissProt: Q16875 Human Unigene: 444304 Human Unigene: 44844 Rat |
| 產品圖片 |
Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti- Phospho-PFKFB3/PFK2 (Ser467) (bs-3331R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PFKFB3 PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (bs-3331R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PFKFB3/PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (bs-3331R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PFKFB3/PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (bs-3331R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
