| 產品編號 | bsm-1625M |
| 英文名稱 | PSA(Pa3) Mouse mAb |
| 中文名稱 | 人前列腺特異性抗原單克隆抗體(包被) |
| 別 名 | antigen, prostate-specific; APS; gamma seminoprotein; gamma-seminoprotein; hK3; Kallikrein 3; Kallikrein 3, (prostate specific antigen); Kallikrein related peptidase 3; Kallikrein-3; KLK 3; KLK2A1; KLK3; KLK3_HUMAN; P 30 antigen; P-30 antigen; P30 antigen; Prostate Specific Antigen; Prostate-specific antigen; PSA; Semenogelase; Seminin. |
 |
Specific References (4) | bsm-1625M has been referenced in 4 publications.
[IF=7.243] Fanchun Zeng. et al. Antagonizing exosomal miR-18a-5p derived from prostate cancer cells ameliorates metastasis-induced osteoblastic lesions by targeting Hist1h2bc and activating Wnt/β-catenin pathway. GENES DIS. 2022 Jul;: IHC ; Mouse.
[IF=5.401] Liu, Yingshuai, Lingli Lei, and Zeying Zhang. "An ultrasensitive colorimetric immunoassay based on glucose oxidase catalyzed cascade formation of blue–black iodine–starch complex." Sensors and Actuators B: Chemical (2017).
[IF=4.71] Liu, Yingshuai, et al. "A concentration? dependent multicolor conversion strategy for ultrasensitive colorimetric immunoassay with the naked eye." Analytica Chimica Acta (2017). ELISA ; Rabbit.
[IF=4.04] Zhou, Jie, et al. "Spectrum-based and color-selective electrochemiluminescence immunoassay for determining human prostate specific antigen in near-infrared region." Talanta 165 (2017): 117-121. other ; Human.
|
| 研究領域 | 腫瘤 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | Pa3 |
| 交叉反應 | Human |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | ≥1mg/ml |
| 免 疫 原 | Full length native protein purified from human: full length |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Function: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Subunit: Forms heterodimer with SERPINA5. Subcellular Location: Secreted. Tissue Specificity: Prostate. Similarity: Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain. SWISS: P07288 Gene ID: 354 Database links: Entrez Gene: 354 Human Omim: 176820 Human SwissProt: P07288 Human Unigene: 171995 Human PSA-前列腺特異性抗原為激肽釋放酶家族蛋白，為絲氨酸蛋白酶，由前列腺上皮產生，精液中的濃度很高，可使精液凝塊水解、液化，功能與男性生育力有關. 此抗體可用于試劑盒包被用抗體。 |
| 產品圖片 |
Tissue/cell: human prostate carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PSA(Pa3) Polyclonal Antibody, Unconjugated(bsm-1625M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
