| 產品編號 | bs-1603R |
| 英文名稱 | AARS2 Rabbit pAb |
| 中文名稱 | 丙氨酰tRNA合成酶2抗體 |
| 別 名 | alanyl tRNA synthetase 2; AARSL; bA444E17.1; Gm89; MT ALARS; MTALARS; AARS 2; AARS2; Alanine tRNA ligase 2 mitochondrial; Alanine tRNA ligase; Alanyl tRNA synthetase 2 mitochondrial; Alanyl tRNA synthetase like; AlaRS; KIAA1270; MGC69820; Probable alanyl tRNA synthetase mitochondrial; RP11-444E17.1. |
| 研究領域 | 細胞生物 信號轉導 細胞凋亡 激酶和磷酸酶 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Dog) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 115 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AARS2: 451-550/985 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play critical roles in mRNA translation by charging tRNAs with their cognate amino acids. The encoded protein is a mitochondrial enzyme that specifically aminoacylates alanyl-tRNA. Mutations in this gene are a cause of combined oxidative phosphorylation deficiency 8. [provided by RefSeq, Dec 2011]. Function: The human alanyl-tRNA synthetase (AlaRS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AlaRS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. Alanyl-tRNA synthetase (AlaRS) catalyzes the ligation of alanine with alanyl-tRNA using ATP. Subcellular Location: Mitochondrion matrix. SWISS: Q5JTZ9 Gene ID: 57505 Database links: Entrez Gene: 57505 Human Omim: 612035 Human SwissProt: Q5JTZ9 Human |
| 產品圖片 |
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Lane 3: Lung (Mouse) Lysate at 40 ug
Lane 4: Spleen (Mouse) Lysate at 40 ug
Lane 5: Liver (Mouse) Lysate at 40 ug
Lane 6: Cerebrum (Rat) Lysate at 40 ug
Lane 7: Cerebellum (Rat) Lysate at 40 ug
Lane 8: Lung (Rat) Lysate at 40 ug
Lane 9: Spleen (Rat) Lysate at 40 ug
Lane 10: Liver (Rat) Lysate at 40 ug
Lane 11: 293T (Human) Cell Lysate at 30 ug
Lane 12: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-AARS2 (bs-1603R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kD
Observed band size: 110 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
