| 產品編號 | bs-1333R |
| 英文名稱 | TFAR19/PDCD5 Rabbit pAb |
| 中文名稱 | 凋亡相關蛋白5抗體 |
| 別 名 | TF-1 cell apoptsis-related gene 19; Programmed cell death protein 5; TF-1 cell apoptosis-related protein 19; Protein TFAR19; PDCD5. |
| 研究領域 | 腫瘤 細胞生物 發(fā)育生物學 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse,Cow,Chicken,Dog) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TFAR19: 45-125/125 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a protein expressed in tumor cells during apoptosis independent of the apoptosis-inducing stimuli. Prior to apoptosis induction, this gene product is distributed in both the nucleus and cytoplasm. Once apoptosis is induced, the level of this protein increases and by relocation from the cytoplasm, it accumulates in the nucleus. Although its exact function is not defined, this protein is thought to play an early and universal role in apoptosis. Tissue Specificity: Widely expressed. Highest levels in heart, testis, kidney, pituitary gland, adrenal gland and placenta. Similarity: Belongs to the PDCD5 family. SWISS: O14737 Gene ID: 9141 Database links: Entrez Gene: 9141 Human Entrez Gene: 100042424 Mouse Entrez Gene: 56330 Mouse Omim: 604583 Human SwissProt: O14737 Human SwissProt: P56812 Mouse Unigene: 443831 Human Unigene: 371609 Mouse Unigene: 440265 Mouse 凋亡相關蛋白TFAR19又稱PDCD5，是新近發(fā)現(xiàn)的與促凋亡有關的凋亡相關蛋白，目前主要用于腫瘤細胞凋亡方面的研究。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDCD5) Polyclonal Antibody, Unconjugated (bs-1333R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
