| 產品編號 | bs-1034R |
| 英文名稱 | SP-B/Surfactant Protein B Rabbit pAb |
| 中文名稱 | 肺表面活性蛋白B抗體 |
| 別 名 | 18 kDa pulmonary surfactant protein; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant apoprotein PSP B; Pulmonary surfactant associated protein B 18kD; Pulmonary surfactant associated protein B; Pulmonary surfactant associated prot |
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Specific References (1) | bs-1034R has been referenced in 1 publications.
[IF=1.19] Kanie et al. Suppressive effects of the expectorant drug ambroxol hydrochloride on quartz-induced lung inflammation in F344 rats. (2017) J.Toxicol.Pathol. 30:153-159 IHC-P ; Rat.
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| 研究領域 | 腫瘤 心血管 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse SP-B: 291-377/377 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.Homodimer; disulfide-linked. Secreted, extracellular space. Function: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter. Subunit: Homodimer; disulfide-linked. Subcellular Location: Secreted, extracellular space, surface film. Similarity: Contains 1 saposin A-type domain. Contains 3 saposin B-type domains. SWISS: P50405 Gene ID: 20388 Database links: Entrez Gene: 6439 Human Entrez Gene: 20388 Mouse Omim: 178640 Human SwissProt: P07988 Human SwissProt: P50405 Mouse Unigene: 512690 Human Unigene: 46033 Mouse 肺泡表面活性物質B（SP-B）也屬糖結合蛋白家族,參與肺泡表面活性膜的形成和代謝,除在Ⅱ型細胞中強烈表達外，在細支氣管、支氣管上皮內也有灶性表達。SP-B是疏水性蛋白，主要效應是促進磷脂吸附和分布到肺泡氣-液交界面，促進磷脂單分子層的形成，只有這種單分子層磷脂才能使表面張力降低到最低水平。 |
| 產品圖片 |
Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Lung (Rat) Lysate at 40 ug
Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-SP-B/Surfactant Protein B (bs-1034R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
