| 產(chǎn)品編號 | bs-1183R |
| 英文名稱 | IL17A Rabbit pAb |
| 中文名稱 | 白介素-17抗體 |
| 別 名 | Interleukin-17A; CTLA-8; CTLA 8; CTLA8; Cytotoxic T lymphocyte associated antigen 8; Cytotoxic T lymphocyte associated serine esterase 8; IL 17A; IL-17A; IL-17; Interleukin 17; Interleukin-17; Interleukin 17A; Interleukin17; Interleukin17A; IL17_HUMAN; IL17_MOUSE. |
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Specific References (33) | bs-1183R has been referenced in 33 publications.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | 15-17 |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse IL-17: 85-150/150 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is a member of the IL-17 receptor family which includes five members (IL-17RA-E) and the encoded protein is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. The encoded protein elicits crucial impacts on host defense, cell trafficking, immune modulation, and tissue repair, with a key role in the induction of innate immune defenses. This cytokine stimulates non-hematopoietic cells and promotes chemokine production thereby attracting myeloid cells to inflammatory sites. This cytokine also regulates the activities of NF-kappaB and mitogen-activated protein kinases and can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). IL-17A plays a pivotal role in various infectious diseases, inflammatory and autoimmune disorders, and cancer. High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The lung damage induced by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL17A. [provided by RefSeq, Sep 2020] Function: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Subunit: Homodimer. Subcellular Location: Secreted. Tissue Specificity: Restricted to a subset of activated T-cells. Post-translational modifications: Found both in glycosylated and nonglycosylated forms. Similarity: Belongs to the IL-17 family. SWISS: Q62386 Gene ID: 16171 Database links: Entrez Gene: 3605 Human Entrez Gene: 16171 Mouse Omim: 603149 Human SwissProt: Q16552 Human SwissProt: Q62386 Mouse Unigene: 41724 Human Unigene: 5419 Mouse Unigene: 218513 Rat
白細胞介素17（IL-17A）是一種來源于活化T淋巴細胞的細胞因子,具有較廣泛的生物學活性,它能夠通過誘導基質(zhì)細胞產(chǎn)生前炎性細胞因子和造血細胞因子參與機體免疫調(diào)節(jié)及部分炎癥反應，因此白細胞介素IL-17A可能是某些疾病發(fā)生發(fā)展的重要因素之一。Interleukin-17 原稱CTLA-8, 是T細胞表達的多肽，參與造血干系統(tǒng)的調(diào)節(jié)，已經(jīng)顯示IL-17促進纖維母細胞產(chǎn)生IL-6，IL-8和G-CSF。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Rat Lymph node tissue lysates
Lane 2: Mouse Lymph node tissue lysates
Lane 3: Rat Thymus tissue lysates
Primary: Anti-IL17A (bs-1183R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 22 kD
Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-17/IL-17A Polyclonal Antibody, Unconjugated(bs-1183R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-17/IL-17A Polyclonal Antibody, Unconjugated(bs-1183R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
