| 產品編號 | bs-0394R |
| 英文名稱 | HOXc8 Rabbit pAb |
| 中文名稱 | HOXc8抗體 |
| 別 名 | Hox-3.1; Homeo box 3A; Homeo box 8; Homeobox 3A; Homeobox C8; Homeobox protein Hox C8; Homeobox protein Hox-C8; Homeobox protein HoxC8; Homeobox protein R4; Homolog of mouse Hox 3.1; HOX 3; Hox 3.1 mouse homolog of; HOX 3A; HOX C8; Hox-3.1, MOUSE, HOMOLOG OF; Hox-3A; HOX3; HOX3A; HOXC 8; Hoxc-8; M31; HXC8_HUMAN. |
| 研究領域 | 免疫學 神經生物學 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Pig,Cow,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HOXc8: 51-130/242 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The product of this gene may play a role in the regulation of cartilage differentiation. It could also be involved in chondrodysplasias or other cartilage disorders. Function: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Subunit: Interacts with SMAD1 and HOMEZ. Subcellular Location: Nucleus. Similarity: Belongs to the Antp homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: P31273 Gene ID: 3224 Database links: Entrez Gene: 3224 Human Entrez Gene: 15426 Mouse Omim: 142970 Human SwissProt: P31273 Human SwissProt: P09025 Mouse Unigene: 664500 Human Unigene: 6167 Mouse HOXc8基因是HOX基因家族中的一員，研究小鼠中HOXc8蛋白的位置、結構以及HOXc8蛋白在小鼠胚胎發育中的表達與調控、小鼠神經系統的發育均已被廣泛重視. HOXc8蛋白的表達對APP基因的表達有抑制作用,有學者認為HOXc8基因可能與Alzheimer's病的發病有關. 目前對哺乳動物同源盒基因在中樞神經系統發育中的廣泛表達以及在胚胎發育中的區域特異性表達方式被認為是非常重要的，已引起科學家們的廣泛重視。 |
| 產品圖片 |
Sample:
Brain(Rat) lysate at 30ug;
Liver(Rat) lysate at 30ug;
Primary: Anti-HOXc8 (bs-0394R) at 1:200;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000;
Predicted band size : 27kD
Observed band size : 27kD, 43kD
Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti-HOXc8 ? (bs-0394R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3) Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37°C for 30min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
