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產品編號	bs-0287R
英文名稱	Rabbit Anti-His tag antibody
中文名稱	His tag標簽抗體
別名	HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
	Specific References (17) \| bs-0287R has been referenced in 17 publications. [IF=2.914] WB ; Pig. PubMed:32649964 [IF=19.924] Yifan Yang. et al. A Versatile Platform for the Tumor-Targeted Intracellular Delivery of Peptides, Proteins, and siRNA. ADV FUNCT MATER. 2023 Apr;:2301011 WB ; Human. PubMed:10.1002/adfm.202301011 [IF=7.2] Zhiyan Hu. et al. CIP4 targeted to recruit GTP-Cdc42 involving in invadopodia formation via NF-κB signaling pathway promotes invasion and metastasis of CRC. Mol Ther-Oncolytics. 2022 Feb;: WB ; Strain（Bl21）. PubMed:10.1016/j.omto.2022.02.023 [IF=4.38] Ma, Xiangyi, et al. "DT390-triTMTP1, a novel fusion protein of diphtheria toxin with tandem repeat TMTP1 peptide, preferentially targets metastatic tumors." Molecular pharmaceutics 10.1 (2013): 115-126. IHC-P ; PubMed:23198829 [IF=4.235] Xu Zhenshang. et al. Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases. Front Microbiol. 2020 Nov;11:2281 WB ; PubMed:33329424 [IF=3.309] Chuandong Cheng. et al. SREBP2/Rab11s/GLUT1/6 network regulates proliferation and migration of glioblastoma. PATHOL RES PRACT. 2022 Oct;:154176 WB ; Human. PubMed:36327817 [IF=2.984] Ruirui Luoet al. Clostridium perfringens beta2 toxin induced in vitro oxidative damage and its toxic assessment in porcine small intestinal epithelial cell lines. Gene . 2020 Oct 30;759:144999. WB ; pig. PubMed:32717305 [IF=2.984] WB, IF ; Pig. PubMed:32717305 [IF=2.914] Xiaoli Gao. et al. Effects of Clostridium perfringens beta2 toxin on apoptosis, inflammation, and barrier function of intestinal porcine epithelial cells. Microb Pathogenesis. 2020 Oct;147:104379 WB ; Pig. PubMed:32649964 [IF=2.913] Li N et al. Identification and characterization of an atypical RIG-I encoded by planarian Dugesia japonica and its essential role in the immune response.(2019) Dev Comp Immunol. Feb;91:72-84. WB ; PubMed:30355517 [IF=2.7] Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013). WB ; PubMed:Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gen [IF=2.7] WANG, Peng, et al. "Construction and identification of a recombinant adenovirus vector expressing His-tag-ICP47 fusion gene." Life Science Journal 9.1 (2012). PubMed:Life Science Journal 9.1 (2012) [IF=2.56] Wang, P. et al. "Attenuation of cytokine production of interleukine -2 and perforin in Two-way mixed lymphocyte reaction by highly efficient transduction of the recombinant adenovirus expressing ICP47 gene."Life Sci J 2013;10(3):963-969 WB ; PubMed:ISSN:1097-8135 [IF=1.74] Wang, Peng, et al. "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." Cellular Immunology (2013). WB ; PubMed:23774531 [IF=1.676] Ma et al. Adjuvant effects mediated by the carbohydrate recognition domain of Agrocybe aegerita lectin interacting with avian influenza H9N2 viral surface glycosylated proteins. (2017) J.Zhejiang.Univ.Sci.. 18:653-661 other ; PubMed:28786240 [IF=1.109] He,et al.Recombinant Fiber-2 Protein-Based Indirect ELISA for Antibody Detection of Fowl Adenovirus Serotype 4.(2018) Avian Diseases. 62:73-78. WB ; PubMed:29620471 [IF=0] Ren et al. Long-term rescue of rat retinal ganglion cells and visual function by AAV-mediated BDNF expression after acute elevation of intraocular pressure. (2012) Invest.Ophthalmol.Vis.Sci. 53:1003-11 IHC ; PubMed:22247466
產品類型	標簽抗體
研究領域	腫瘤細胞生物免疫學染色質和核信號信號轉導合成與降解結合蛋白
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應	Recombinant protein
產品應用	WB=1:2000-5000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
性狀	Liquid
濃度	1mg/ml
免疫原	Synthetic peptide: HHHHHH conjugated to KLH
亞型	IgG
純化方法	affinity purified by Protein A
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產品介紹	The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. SWISS: N/A Gene ID: N/A 標簽抗體蛋白質純化的新途徑─應用His-Tag抗體檢測His-Tag融合蛋白 1） 6xHis與不同的Sepharose純化填料結合（捆綁），可不受構象的影響，在天然或變形的條件下可進行進一步純化。 2）可在溫和的洗脫液環境中，其結合、洗滌及洗脫步驟不會對蛋白結構產生影響。 3） 6xHis標簽比普通（Tag）要小得多，經純化后的蛋白可直接進行下游操作。可用于任何表達系統（包括：細菌，桿狀病毒和哺乳動物） 4） 6xHis標簽在生理溶液pH下不帶電荷，其標簽不會干涉重組蛋白的結構和功能， 6xHis不會影響蛋白分泌 5） 6xHis標簽免疫原性差，重組蛋白可以不必除去標簽而直接作為抗原進行免疫
產品圖片	Sample: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4 ug Primary: Anti-His Tag (bs-0287R) at 1/2000 ~ 1/5000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標記二抗

	1、抗體溶解方法
	2、抗體修復方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關于肽鏈的設計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序

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