| 產品編號 | bsm-63003R |
| 英文名稱 | Thioredoxin Recombinant Rabbit mAb |
| 中文名稱 | 硫氧還蛋白重組兔單抗 |
| 別 名 | TXN; ADF; ATL-derived factor; SASP; Thioredoxin; TRX1; Thioredoxin delta 3; TRX; TXN delta 3; TRDX. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 2D20 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12 kDa |
| 檢測分子量 | 12 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Thioredoxin: 1-55 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. SWISS: P10599 Gene ID: 7295 硫氧還蛋白(Thioredoxin，Trx)是一類廣泛存在于生物體內的多功能酸性蛋白，分子量約12kDa，是一種小分子含硒蛋白質,參與細胞的一系列生化反應，包括酶活性的調節、轉錄因子的調控等，是重要的酶活力調節蛋白。 硫氧還蛋白的功能絕大多數是依賴于硫氧還蛋白還原靶蛋白中的二硫鍵。所有硫氧還蛋白均有一個保守的活性中心，Trp-Cys-Gly(Ala)-Pro-Cys，活性中心有兩個具有氧還活性的半胱氨酸殘基。 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Thioredoxin monoclonal antibody, unconjugated (bsm-63003R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Thioredoxin) monoclonal Antibody, unconjugated (bsm-63003R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
