| 產品編號 | bsm-62990R |
| 英文名稱 | TFF3 Recombinant Rabbit mAb |
| 中文名稱 | 三葉肽因子3重組兔單抗 |
| 別 名 | hITF; hP1.B; Intestinal trefoil factor; ITF; mITF; P1B; Polypeptide P1.B; TFF3; TFI; TREFOIL; Trefoil factor 3. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 9 kDa |
| 檢測分子量 | 9 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Trefoil factor 3: 50-80/80 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). SWISS: Q07654 Gene ID: 7033 三葉草肽Ⅲ是三葉肽中結構獨特的一類小分子肽,它對于胃黏膜保護及損傷后修復具有重要作用,它的作用機制復雜,通過與其受體作用機制及信號傳導機制的研究,以加深其對胃黏膜保護機制的認識。在促進黏膜愈合的治療上,三葉肽是一個新的思路。 局部應用三葉肽可能使黏膜凝膠產生更大的穩定性,使胃黏膜對各種損傷因子的防御能力增強。三葉肽在誘導上皮修復上的直接作用,可用于許多胃腸道疾病。它作為輔助劑或取代皮質類固醇或其他免疫抑制劑，在潰瘍病及炎癥性腸病的治療上可能是一個突破。三葉肽研究可能對胃十二指腸潰瘍和炎癥、壞死性小腸結腸炎 |
| 產品圖片 |
Western blot analysis of human small intestine lysate. Using TFF3 (bsm-62990R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TFF3 Monoclonal Antibody, Unconjugated(bsm-62990R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TFF3 Monoclonal Antibody, Unconjugated(bsm-62990R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TFF3 Monoclonal Antibody, Unconjugated(bsm-62990R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TFF3 Monoclonal Antibody, Unconjugated(bsm-62990R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TFF3 Monoclonal Antibody, Unconjugated(bsm-62990R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
