| 產品編號 | bsm-61121R |
| 英文名稱 | Adiponectin Recombinant Rabbit mAb |
| 中文名稱 | 脂聯素重組兔單抗 |
| 別 名 | 30 kDa adipocyte complement related protein; ACDC; ACRP30; Adipocyte; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; adipocyte-specific secretory protein; Adiponectin; Adiponectin precursor; adiponectin, C1Q and collagen domain containing; Adipoq; Adipose most abundant gene transcript 1; Adipose specific collagen like factor; ADIPQTL1; ADPN; APM1; C1q and collagen domain-containing protein; GBP28; Gelatin binding protein; Gelatin binding protein 28. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 11F6 |
| 交叉反應 | Mouse |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測分子量 | 28 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from mouse Adiponectin: 1-247 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. SWISS: Q60994 Gene ID: 11450 脂聯素又被稱作Acrp30、apM1、AdipoQ、GBP28。 最初, 脂聯素是在人體皮下脂肪組織、血漿和鼠科動物的脂肪細胞中被發現人體內的脂聯素由244個氨基酸組成,分子量為27KD。 脂聯素影響脂肪和糖類的代謝，還可以降低多種粘附分子(VCAM-1、ICAM-1、選擇素-E) 在內皮細胞的表達。并影響著腫瘤壞死因子（TNF）分泌，近年來主要用于糖尿病的研究。 脂聯素作為一種胰島素超敏化激素(An Insulin–sensitizing Hormone),可以增加促進骨骼肌細胞的脂肪酸氧化和糖吸收,明 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Adiponectin monoclonal antibody, unconjugated (bsm-61121R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Adiponectin Monoclonal Antibody, Unconjugated (bsm-61121R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Adiponectin Monoclonal Antibody, Unconjugated (bsm-61121R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Adiponectin Monoclonal Antibody, Unconjugated (bsm-61121R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
