| 產品編號 | bsm-61120R |
| 英文名稱 | GM130 Recombinant Rabbit mAb |
| 中文名稱 | 高爾基體自身蛋白GM130重組兔單抗 |
| 別 名 | GM130; Gm130 autoantigen; GOLGA 2; Golga2; Golgi autoantigen; Golgin 95. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Cow,Dog,Monkey) |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 113 kDa |
| 檢測分子量 | 130 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Golgin subfamily A member 2: 1-57 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. SWISS: Q08379 Gene ID: 2801 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with GM130 monoclonal antibody, unconjugated (bsm-61120R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
